Journal: Regenerative Biomaterials

Article Title: Temporal control in shell–core structured nanofilm for tracheal cartilage regeneration: synergistic optimization of anti-inflammation and chondrogenesis

doi: 10.1093/rb/rbae040

Figure Lengend Snippet: In vitro anti-inflammatory assessment of the PLGA@ICA-GT@KGN nanofilm on LPS-stimulated RAW264.7 macrophages. ( A ) Inflammatory-related protein expression (IL-1β, IL-6 and TNF-α) in different groups—control, PLGA-GT, PLGA@ICA-GT, PLGA-GT@KGN and PLGA@ICA-GT@KGN—is examined after 24 h of in vitro coculture via WB examination. ( B ) Inflammatory-related gene expression ( IL-1β , IL-6 and TNF-α ) in various groups after 24 h of in vitro co-culture is analyzed using qRT-PCR (* P < 0.05). ( C ) Immunofluorescence staining for IL-6 and TNF-α in various groups after 24 h of in vitro co-culture.

Article Snippet: The P2 BMSCs were collected and subjected to immunofluorescence staining to detect the expression of antigens such as CD44, CD90 and CD34, following the instructions of the immunofluorescence detection kit (Beijing Baiao Leibo Technology Co., Ltd, China).

Techniques: In Vitro, Expressing, Control, Gene Expression, Co-Culture Assay, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Regenerative Biomaterials

Article Title: Temporal control in shell–core structured nanofilm for tracheal cartilage regeneration: synergistic optimization of anti-inflammation and chondrogenesis

doi: 10.1093/rb/rbae040

Figure Lengend Snippet: In vitro chondrogenic assessment of pretreated PLGA@ICA-GT@KGN nanofilm on BMSCs. ( A ) Immunofluorescence staining for COL II of BMSC-loaded nanofilms is depicted across different groups—control, PLGA-GT, PLGA@ICA-GT, PLGA-GT@KGN and PLGA@ICA-GT@KGN—after 14 days of in vitro culture. ( B ) Immunofluorescence staining for SOX9 of BMSC-loaded nanofilms is presented across the same groups after 14 days of in vitro culture. ( C – E ) Gene expression levels of chondrogenic-related markers ( SOX9 , ACAN and Col2a1 ) in various groups are examined after 14 days of in vitro culture. ( F , G ) Quantitative analysis of GAG, COL II and total collagen contents in different groups is conducted after 14 days of in vitro culture (* P < 0.05).

Article Snippet: The P2 BMSCs were collected and subjected to immunofluorescence staining to detect the expression of antigens such as CD44, CD90 and CD34, following the instructions of the immunofluorescence detection kit (Beijing Baiao Leibo Technology Co., Ltd, China).

Techniques: In Vitro, Immunofluorescence, Staining, Control, Gene Expression

Journal: Regenerative Biomaterials

Article Title: Temporal control in shell–core structured nanofilm for tracheal cartilage regeneration: synergistic optimization of anti-inflammation and chondrogenesis

doi: 10.1093/rb/rbae040

Figure Lengend Snippet: In vivo anti-inflammatory evaluation. ( A ) A schematic illustrates the preparation of the BMSCs-nanofilm construct using a ‘sandwich model’, which was subsequently employed for repairing circular tracheal defects. ( B ) Immunofluorescence staining for CD3 and CD68 is displayed in samples from the PLGA-GT, PLGA@ICA-GT, PLGA-GT@KGN and PLGA@ICA-GT@KGN groups after 2 weeks of tracheal transplantation. ( C ) Immunofluorescence staining for CD3 and CD68 in the same groups is shown after 4 weeks of tracheal transplantation. The percentages of ( D ) CD3 and ( E ) CD68 positive areas are quantified in the PLGA-GT, PLGA@ICA-GT, PLGA-GT@KGN and PLGA@ICA-GT@KGN groups at 2 and 4 weeks (* P < 0.05).

Article Snippet: The P2 BMSCs were collected and subjected to immunofluorescence staining to detect the expression of antigens such as CD44, CD90 and CD34, following the instructions of the immunofluorescence detection kit (Beijing Baiao Leibo Technology Co., Ltd, China).

Techniques: In Vivo, Construct, Immunofluorescence, Staining, Transplantation Assay